polymac enrichment kit Search Results


polymac ti kit  (Tymora Analytical Operations LLC)
94
Tymora Analytical Operations LLC polymac ti kit
Polymac Ti Kit, supplied by Tymora Analytical Operations LLC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymac+enrichment+kit/pm30103608-120-5-7?v=Tymora+Analytical+Operations+LLC
Average 94 stars, based on 1 article reviews
polymac ti kit - by Bioz Stars, 2026-08
94/100 stars
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evtrap  (Tymora Analytical Operations LLC)
94
Tymora Analytical Operations LLC evtrap
Evtrap, supplied by Tymora Analytical Operations LLC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymac+enrichment+kit/custom%40evtrp%4037576973?v=Tymora+Analytical+Operations+LLC
Average 94 stars, based on 1 article reviews
evtrap - by Bioz Stars, 2026-08
94/100 stars
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magnetic separator rack  (Promega)
90
Promega magnetic separator rack
Magnetic Separator Rack, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymac+enrichment+kit/pmc06125197-512-92-95?v=Promega
Average 90 stars, based on 1 article reviews
magnetic separator rack - by Bioz Stars, 2026-08
90/100 stars
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pts lysis solution see recipe ammonium bicarbonate  (Thermo Fisher)
99
Thermo Fisher pts lysis solution see recipe ammonium bicarbonate
Pts Lysis Solution See Recipe Ammonium Bicarbonate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymac+enrichment+kit/pmc06125197-402-5-22?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
pts lysis solution see recipe ammonium bicarbonate - by Bioz Stars, 2026-08
99/100 stars
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lys-c (optional) – lysis endopeptidase  (FUJIFILM)
90
FUJIFILM lys-c (optional) – lysis endopeptidase
Lys C (Optional) – Lysis Endopeptidase, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymac+enrichment+kit/pmc06125197-439-30-35?v=FUJIFILM
Average 90 stars, based on 1 article reviews
lys-c (optional) – lysis endopeptidase - by Bioz Stars, 2026-08
90/100 stars
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np 40 lysis solution  (Thermo Fisher)
99
Thermo Fisher np 40 lysis solution
Phosphoproteomic data comparison between standard overnight digestion in urea, 1-hour long digestion using on-beads Smart Digest trypsin, and 1-hour long digestion using soluble Smart Digest trypsin. Phosphopeptide and nonphosphopeptide identification numbers for each are shown. Each sample started with 100 μg of cell culture proteins extracted using <t>NP-40</t> lysis solution and analyzed by a 60-min LC-MS gradient.
Np 40 Lysis Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymac+enrichment+kit/pmc06125197-648-4-14?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
np 40 lysis solution - by Bioz Stars, 2026-08
99/100 stars
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bicinchoninic acid bca protein assay kit  (Thermo Fisher)
99
Thermo Fisher bicinchoninic acid bca protein assay kit
Troubleshooting for Phosphoproteomic Experiments
Bicinchoninic Acid Bca Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymac+enrichment+kit/pmc06125197-512-26-32?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
bicinchoninic acid bca protein assay kit - by Bioz Stars, 2026-08
99/100 stars
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lys-c  (FUJIFILM)
90
FUJIFILM lys-c
Troubleshooting for Phosphoproteomic Experiments
Lys C, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymac+enrichment+kit/pmc06125197-69-38-43?v=FUJIFILM
Average 90 stars, based on 1 article reviews
lys-c - by Bioz Stars, 2026-08
90/100 stars
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pipette bulb centrifugal vacuum concentrator  (Labconco)
99
Labconco pipette bulb centrifugal vacuum concentrator
Troubleshooting for Phosphoproteomic Experiments
Pipette Bulb Centrifugal Vacuum Concentrator, supplied by Labconco, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymac+enrichment+kit/pmc06125197-439-86-91?v=Labconco
Average 99 stars, based on 1 article reviews
pipette bulb centrifugal vacuum concentrator - by Bioz Stars, 2026-08
99/100 stars
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Image Search Results


Phosphoproteomic data comparison between standard overnight digestion in urea, 1-hour long digestion using on-beads Smart Digest trypsin, and 1-hour long digestion using soluble Smart Digest trypsin. Phosphopeptide and nonphosphopeptide identification numbers for each are shown. Each sample started with 100 μg of cell culture proteins extracted using NP-40 lysis solution and analyzed by a 60-min LC-MS gradient.

Journal: Current protocols in chemical biology

Article Title: Identification of Phosphorylated Proteins on a Global Scale

doi: 10.1002/cpch.48

Figure Lengend Snippet: Phosphoproteomic data comparison between standard overnight digestion in urea, 1-hour long digestion using on-beads Smart Digest trypsin, and 1-hour long digestion using soluble Smart Digest trypsin. Phosphopeptide and nonphosphopeptide identification numbers for each are shown. Each sample started with 100 μg of cell culture proteins extracted using NP-40 lysis solution and analyzed by a 60-min LC-MS gradient.

Article Snippet: list-behavior=simple prefix-word= mark-type=none max-label-size=0 NP-40 lysis solution (see recipe) Smart Digest Soluble Trypsin kit (Thermo Fisher Scientific) Dithiothreitol (DTT; see recipe) Iodoacetamide (IAA; see recipe) Bicinchoninic Acid (BCA) protein assay kit (Life Technologies, Millipore-Sigma) Trifluoroacetic acid (TFA) Acetonitrile Methanol PolyMAC Magnetic phosphopeptide enrichment kit (Tymora Analytical) Formic acid 1.7 mL nonstick microfuge tubes Sonicator probe Centrifuge capable of speed 16,000 × g Thermal shaker capable of reaching 70°C and shaking at 1,200 rpm Microplate reader for Vis range (Biotek, Fisher Scientific) Sep-Pak C18 column (Waters) Pipette bulb Centrifugal vacuum concentrator (Labconco) Magnetic separator rack (Promega, VWR) Cell lysis list-behavior=simple prefix-word= mark-type=none max-label-size=1 1 Obtain a cell pellet (keep at −80°C until ready to lyse). list-behavior=simple prefix-word= mark-type=none max-label-size=0 If an already extracted protein sample is used (e.g. previously lysed in compatible buffer, IP sample, plasma, CSF, etc.), start the procedure at step 7.

Techniques: Cell Culture, Lysis, Liquid Chromatography with Mass Spectroscopy

Troubleshooting for Phosphoproteomic Experiments

Journal: Current protocols in chemical biology

Article Title: Identification of Phosphorylated Proteins on a Global Scale

doi: 10.1002/cpch.48

Figure Lengend Snippet: Troubleshooting for Phosphoproteomic Experiments

Article Snippet: Materials list-behavior=simple prefix-word= mark-type=none max-label-size=0 NP-40 lysis solution (see recipe) Smart Digest Soluble Trypsin kit (Thermo Fisher Scientific) Dithiothreitol (DTT; see recipe) Iodoacetamide (IAA; see recipe) Bicinchoninic Acid (BCA) protein assay kit (Life Technologies, Millipore-Sigma) Trifluoroacetic acid (TFA) Acetonitrile Methanol PolyMAC Magnetic phosphopeptide enrichment kit (Tymora Analytical) Formic acid 1.7 mL nonstick microfuge tubes Sonicator probe Centrifuge capable of speed 16,000 × g Thermal shaker capable of reaching 70°C and shaking at 1,200 rpm Microplate reader for Vis range (Biotek, Fisher Scientific) Sep-Pak C18 column (Waters) Pipette bulb Centrifugal vacuum concentrator (Labconco) Magnetic separator rack (Promega, VWR) Cell lysis list-behavior=simple prefix-word= mark-type=none max-label-size=1 1 Obtain a cell pellet (keep at −80°C until ready to lyse). list-behavior=simple prefix-word= mark-type=none max-label-size=0 If an already extracted protein sample is used (e.g. previously lysed in compatible buffer, IP sample, plasma, CSF, etc.), start the procedure at step 7.

Techniques: Lysis, Solubility, BIA-KA